MARROW GRANULOCYTE-MACROPHAGE PROGENITOR CELL RESPONSE TO BURN INJURY AS MODIFIED BY ENDOTOXIN AND INDOMETHACIN

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Abstract
The production and release of granulocytes and macrophages are significantly impaired following burn injury and infection. In an attempt to determine the factors responsible for these adverse effects and their potential treatments, we performed a series of studies in mice analyzing the bone marrow response to burn wound infection. The proliferative response of the marrow granulocyte-macrophage progenitor cell (GM-CFC) in male BDF1 mice undergoing a dorsal scald burn or burn wound seeding with 1000 colony forming units of Pseudomonas aeruginosa was determined on day 3 postburn using a clonal culture of GM-CFC. Mice with infected burn wounds had a rate of GM-CFC proliferation that was 50% that of noninfected animals and levels of circulating colony stimulating activity (CSA) 30% those of controls (p = 0.006). Similar suppression of marrow proliferative status could be replicated with the administration of endotoxin to normal or burned animals as had been observed for burn-infected animals. The administration of indomethacin (5 mg/kg.day) substantially restored the GM-CFC proliferation in mice with infected burns as well as in animals given endotoxin. Indomethacin-treated animals had CSA values 244% those of untreated burn-infected animals (p = 0.016). We take these observations to suggest that suppression of myelopoiesis in burn-infected animals is related in part to endotoxin-stimulated production of prostaglandin mediators that altered myeloid proliferation and was responsive to cyclooxygenase blockade.

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