Action of the Thiamine Antagonist Bacimethrin on Thiamine Biosynthesis
- 1 October 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (19) , 5606-5610
- https://doi.org/10.1128/jb.182.19.5606-5610.2000
Abstract
Bacimethrin is an analog of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine and inhibits the growth of Salmonella enterica serovar Typhimurium on a defined medium. Two classes of mutants that had increased bacimethrin resistance were isolated and characterized. Results showed that overexpression of the thioperon or specific lesions in thiD resulted in a bacimethrin-resistant phenotype. Phenotypic analyses of thethiD mutants suggested that they had a specific defect in one of the two kinase activities associated with this gene product and, further, that ThiD and not PdxK was primarily responsible for salvage of HMP from the medium.Keywords
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