Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations
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Open Access
- 3 March 2003
- journal article
- review article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 160 (5) , 629-633
- https://doi.org/10.1083/jcb.200210140
Abstract
The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.Keywords
This publication has 25 references indexed in Scilit:
- Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDINature Cell Biology, 2002
- GFP imaging: methodology and application to investigate cellular compartmentation in plantsJournal of Experimental Botany, 2001
- Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cellTrends in Cell Biology, 1999
- Visualization of Pit-1 Transcription Factor Interactions in the Living Cell Nucleus by Fluorescence Resonance Energy Transfer MicroscopyMolecular Endocrinology, 1998
- Diffusional Mobility of Golgi Proteins in Membranes of Living CellsScience, 1996
- Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transferCurrent Biology, 1996
- Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation.The Journal of cell biology, 1995
- Fluorescence ratio imaging of cyclic AMP in single cellsNature, 1991
- Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluationJournal of Muscle Research and Cell Motility, 1987
- Use of resonance energy transfer to monitor membrane fusionBiochemistry, 1981