Effective immunization against cutaneous leishmaniasis with defined membrane antigens reconstituted into liposomes.
Open Access
- 15 February 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 140 (4) , 1274-1279
- https://doi.org/10.4049/jimmunol.140.4.1274
Abstract
The abundant Leishmania promastigote surface Ag gp63 and Leishmania promastigote lipophosphoglycan were reconstituted into liposomes and used as a vaccine against the agent of New World cutaneous leishmaniasis, Leishmania mexicana. The Ag were inoculated s.c., i.p., and i.v. into CBA/ca and BALB/c mice. Even at low Ag dosages, 8 to 10 micrograms/mouse, the Ag induced appreciable levels of protection. In CBA/ca mice complete protection was obtained by s.c. inoculation of antigen-containing liposomes. Protection could be transferred with T cells to naive mice. Interestingly, the Ag-containing liposomes did not cause the disease exacerbation observed in previous vaccine studies with crude parasite extracts.This publication has 4 references indexed in Scilit:
- Structure of the lipid moiety of the Leishmania donovani lipophosphoglycan.Journal of Biological Chemistry, 1987
- Parasite-accessory cell interactions in murine leishmaniasis. II. Leishmania donovani suppresses macrophage expression of class I and class II major histocompatibility complex gene products.The Journal of Immunology, 1987
- Higher frequency of Leishmania major-specific L3T4+ T cells in susceptible BALB/c as compared with resistant CBA mice.The Journal of Immunology, 1986
- Surface antigenic change during differentiation of a parasitic protozoan, Leishmania mexicana: Identification by monoclonal antibodies.Proceedings of the National Academy of Sciences, 1982