COMPARISON OF METABOLISM-MEDIATED BINDING TO DNA OF 7-HYDROXYMETHYL-12-METHYLBENZ(A)ANTHRACENE AND 7,12-DIMETHYLBENZ(A)ANTHRACENE
- 1 January 1979
- journal article
- research article
- Vol. 39 (4) , 1154-1158
Abstract
Comparison of the binding to DNA of 7-hydroxymethyl-12-methylbenz(a)anthracene and 7,12-dimethylbenz(a)anthracene (DMBA) catalyzed by mouse embryo cells in culture or by rat liver microsomes indicates that the products formed are different for the 2 hydrocarbons. The hydroxy compound is not an intermediate in the binding of DMBA to DNA in these systems. Binding of the hydroxy compound to DNA in mouse embryo cells is less efficient than for DMBA and is inhibited by 1, 1, 1-trichloropropylene 2,3-oxide, an inhibitor of epoxide hydrase. This and the fluorescence spectra of the hydroxy compound-DNA adducts indicate that the hydroxy compound is activated for DNA binding through the formation of a diol-epoxide in the 1,2,3,4-ring. DMBA apparently is activated through a bay-region diol-epoxide.This publication has 11 references indexed in Scilit:
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