Diagnostic use of an analysis of urinary proteins by a practicable sodium dodecyl sulfate‐electrophoresis method and rapid two‐dimensional electrophoresis

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Abstract
Two methods suitable for routine clinical analyses of urinary proteins are presented and compared. The first is a horizontal sodium dodecyl sulfate‐polyacrylamide gel electrophoresis technique, suitable for simultaneous analysis of 20 native urinary samples. This method uses polyacrylamide gradient gels, prepared with a laboratorybuilt gel casting device. The second method is a rapid two‐dimensional electrophoresis procedure, combining cellulose acetate electrophoresis and sodium dodecyl sulfate‐electrophoresis. The first step uses a routine system (Chemetron), the second separation step followed by staining with Coomassie Brilliant Blue R is performed on the PhastSystem. The resulting two‐dimensional patterns reveal urinary proteins distributed according to the 5‐zone pattern of native proteins (albumin, alpha‐1, alpha‐2, beta, gamma‐globulin) as well as to the logarithm of their molecular weights. Examples of (routine) diagnoses with a special interest in the monitoring of kidney transplant patients are shown.