Reverse Enzyme Immunoassay for the Determination of Lolium perenne IgE Antibodies
- 1 January 1983
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 72 (2) , 184-187
- https://doi.org/10.1159/000234864
Abstract
A microplate enzyme-immunoassay technique for the detection of specific anti-Loliumperenne IgE antibodies is described. This method consists of coating polystyrene wells with goat antihuman IgE (epsilon chain specific) and later selecting the IgE from the patients serum in order to finally reveal the bound specific IgE by means of a crude L. perenne extract conjugated with peroxidase. The optimal conditions necessary to achieve maximum sensitivity and specificity using this system have been studied. After testing 98 sera, the results showed a 97.9% correlation between our technique and the RAST.Keywords
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