Characterization of lysocardiolipin from Acinetobacter sp. HO1-N

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Abstract
Triacyl-lysocardiolipin (triacyl-LCL) and diacyl-LCL were isolated from Acinetobacter sp. HO1-N, and their structures were determined by chemical, physical and enzymatic procedures. Deacylation of triacyl-LCL and diacyl-LCL yielded bis-glycerylphosphorylglycerol. Periodate oxidation of both lysolipids was negative. Diglyceride and 2-monoglyceride resulted from the acetic acid hydrolysis of triacyl-LCL, but 2-monoglyceride was the sole product obtained from diacyl-LCL. Cardiolipin (CL)-specific phospholipase D treatment of triacyl-LCL yielded lysophosphatidylglycerol and phosphatidic acid. Pancreatic [porcine] lipase treatment of CL yielded triacyl-LCL and diacyl-LCL. 31P NMR spectrometry showed 2 resonance peaks separated by 40 Hz for CL, 2 overlapping peaks separated by 14 Hz for triacyl-LCL, and 1 peak for diacyl-LCL. The proportion of lysocardiolipin increased as a function of cell age, representing 2-3% of the total phospholipids in early- and mid-exponential growth, 5-7% in late-exponential growth and 12% in the stationary growth phase.

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