A Colorimetric Method for Determination of Saxitoxin

Abstract
Saxitoxin, the paralytic shellfish poison, can be conveniently determined by colorimetry after hydrogen peroxide oxidation and reaction of the resultant guanidine with biacetyl. This colorimetric method has been used to assay saxitoxin quantitatively at concentrations down to 0.5 μg/ml. Saxitoxin levels in the Alaskan butter clam (Saxidomus gigantea) have also been determined by this test coupled with a preliminary chromatographic cleanup procedure. Key words: saxitoxin, paralytic shellfish poison, colorimetry, Alaskan butter clam (Saxidomus gigantea)

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