PKC‐independent inhibition of glutamate exocytosis by arachidonic acid in rat cerebrocortical synaptosomes
- 16 January 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 296 (3) , 317-319
- https://doi.org/10.1016/0014-5793(92)80312-5
Abstract
In rat cerebrocortical synaptosomes, the addition of 4β‐phorbol dibutyrate (4β‐PDBu) and arachidonic acid enhances and decreases, respectively, the glutamate release evoked by 4‐aminopyridine. Pretreatment of synaptosomes with 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) or preincubation with staurosporine, prevent the stimulatory effect of 4β‐PDBu, but are without effect on the inibitory action of arachidonic acid. Moreover, methyl arachidonate, which is not effective as a PKC activator, also strongly inhibits glutamate exocytosis. These results suggest that PKC is not involved in the inhibition of glutamate release by arachidonic acid.Keywords
This publication has 26 references indexed in Scilit:
- An Ion Channel Locus for the Protein Kinase C Potentiation of Transmitter Glutamate Release from Guinea Pig Cerebrocortical SynaptosomesJournal of Neurochemistry, 1991
- Ca2+-Independent Release of Glutamate During In Vitro Anoxia in Isolated Nerve TerminalsJournal of Neurochemistry, 1991
- Inhibition of Glutamate Release by Arachidonic Acid in Rat Cerebrocortical SynaptosomesJournal of Neurochemistry, 1991
- Staurosporine: An Effective Inhibitor for Ca2+/Calmodulin‐Dependent Protein Kinase IIJournal of Neurochemistry, 1991
- Ca2+-Dependent Regulation of Presynaptic Stimulus-Secretion CouplingJournal of Neurochemistry, 1989
- G-protein βγ-subunits activate the cardiac muscarinic K+-channel via phospholipase A2Nature, 1989
- Inhibition by Cyclic AMP of Phorbol Ester-Potentiated Norepinephrine Release from Guinea Pig Brain Cortical SynaptosomesJournal of Neurochemistry, 1988
- Lipoxygenase metabolites of arachidonic acid as second messengers for presynaptic inhibition of Aplysia sensory cellsNature, 1987
- Studies and Perspectives of Protein Kinase CScience, 1986
- Direct activation of purified protein kinase C by unsaturated fatty acids (oleate and arachidonate) in the absence of phospholipids and Ca2+FEBS Letters, 1985