ANF stimulation of detergent‐dispersed particulate guanylate cyclase from bovine adrenal cortex

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Abstract
Particulate guanylate cyclase from bovine adrenal cortex can be stimulated by ANF. A 2‐fold stimulation of the enzyme was obtained with 100 nM ANF and a half‐maximal stimulation, with a 5 nM dose. The stimulation by ANF persisted for at least 30 min. Various detergents, such as Triton X‐100, Lubrol PX, cholate, CHAPS, digitonin and zwittergent, stimulated several‐fold the activity of particulate guanylate cyclase. However, only Triton X‐100 dispersed particulate guanylate cyclase without affecting its response to ANF. The dose‐response curve of ANF stimulation of the particulate and the Triton X‐100 dispersed enzyme was similar. The dispersion of a fully responsive guanylate cyclase to ANF will help us to uncover the type of interactions between guanylate cyclase and ANF. It will also be used as a first step for the purification of an ANF‐sensitive particulate guanylate cyclase.