Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present
- 1 March 2003
- journal article
- Published by Taylor & Francis in Pathogens and Global Health
- Vol. 97 (2) , 131-137
- https://doi.org/10.1179/000349803125002977
Abstract
A new, rapid assay, based on a single-round, multiplex PCR, can be used to detect Plasmodium falciparum, P. vivax, P. malariae or P. ovale in human blood. The PCR, which targets the conserved 18S small-subunit RNA genes of the parasites, not only permits a malarial infection to be detected but also allows each Plasmodium species present to be identified, even in cases of mixed infection.Keywords
This publication has 22 references indexed in Scilit:
- Usefulness of PCR for Diagnosis of Imported Malaria in PolandEuropean Journal of Clinical Microbiology & Infectious Diseases, 2002
- Detection of malaria parasites by nested PCR in south-eastern, Iran: Evidence of highly mixed infections in Chahbahar districtMalaria Journal, 2002
- Detection of Plasmodium falciparum DNA in PlasmaAnnals of the New York Academy of Sciences, 2001
- PCR‐based ELISA technique for malaria diagnosis of specimens from ThailandTropical Medicine & International Health, 2001
- A field study on malaria prevalence in southeastern Laos by polymerase chain reaction assay.The American Journal of Tropical Medicine and Hygiene, 2001
- Validation and standardisation of nucleic acid amplification technology (NAT) assays for the detection of viral contamination of blood and blood productsJournal of Clinical Virology, 2001
- Severe falciparum malariaTransactions of the Royal Society of Tropical Medicine and Hygiene, 2000
- Plasmodium falciparum and P. Vivax: Factors Affecting Sensitivity and Specificity of PCR-Based Diagnosis of MalariaExperimental Parasitology, 1994
- Accuracy of routine laboratory diagnosis of malaria in the United Kingdom.Journal of Clinical Pathology, 1994
- DNA PROBES FOR MALARIA DIAGNOSISThe Lancet, 1984