NMR studies of [1-2H]glucose metabolism in Zymomonas mobilis
- 1 May 1986
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 157 (1) , 195-202
- https://doi.org/10.1111/j.1432-1033.1986.tb09656.x
Abstract
In complementary experiments the metabolism of [1-2H]glucose in H2O and of unlabelled glucose in 2H2O by Zymomonas mobilis was examined. The utilization of [1-2H]glucose by Z. mobilis was monitored by high-resolution 2H NMR. The deuterium-labelling pattern and stereochemistry of the ethanols produced from the metabolism of [1-2H]glucose and unlabelled glucose in 2H2O were determined by a combination of 13C and 1H NMR and selective enzyme action. The labelling patterns were explained in terms of enzyme mechanisms and stereospecificity, and metabolite enolization.This publication has 25 references indexed in Scilit:
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