Abstract
The value of the phosphatase reaction for controlling the heat treatment of milk is unquestioned, but the accepted method of estimating phosphatase activity, namely, the colorimetric method of Kay using the phenol reagent of Folin, has three disadvantages: (1) The number of estimations that can be performed in a given time is limited. (2) Errors in matching the tints may occur. (3) Samples of milk may be accidentally contaminated with traces of phenol, and this will lead to too high a Lovibond value.

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