An alcohol dehydrogenase ribozyme
- 10 August 2003
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 10 (9) , 713-717
- https://doi.org/10.1038/nsb964
Abstract
We report an RNA molecule that exhibits activity analogous to that of alcohol dehydrogenase (ADH). Directed in vitro evolution was used to enrich nicotinamide adenine dinucleotide (NAD+)–dependent redox-active RNAs from a combinatorial pool. The most active ribozyme in the population forms a compact pseudoknotted structure and oxidizes an alcohol seven orders of magnitude faster than the estimated spontaneous rate. Moreover, this ADH RNA was coupled with a redox relay between NADH and flavin adenine dinucleotide to give a NAD+-regeneration system. Our demonstration of the redox ability of RNA adds support to an RNA-based metabolic system in ancient life.Keywords
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