Methods for decreasing interstitial immunoglobulin in tissue slices and cryostat sections
- 1 July 1983
- journal article
- research article
- Published by Springer Nature in Journal of Molecular Histology
- Vol. 15 (7) , 637-644
- https://doi.org/10.1007/bf01002985
Abstract
To determine whether lymphoid antigens and cellular morphology can be preserved after long-distance transport in buffer or cell culture medium, we stained cryostat sections prepared from human tonsil samples that had been kept at 4°C or 20°C for 24, 48 or 72 h. B-Cell antigens, T-cell antigens, and Ia antigens were well preserved after storage up to 72 h in buffer or medium at 4°C. Interstitial immunoglobulin (Ig) was decreased following all incubation procedures. We then investigated methods to diminish interstitial Ig in cryostat sections, since it would be inconvenient to keep 2–3 mm tissue slices in buffer or medium prior to freezing and subsequent Ig staining. Cryostat sections were air dried or briefly fixed in acetone prior to washing in buffer or medium at 4°C, 20°C or 37°C for 1, 2 or 24 h. Then sections were air dried or washed prior to acetone fixation and immunostaining. A method for washing cryostat sections was developed which diminished interstitial Ig without compromising the quality of immunostaining or cellular detail. These methods are especially useful for studying samples of lymphoid tissue in which the presence of large quantities of interstitial Ig obscures the detection of monotypic Ig staining patterns.This publication has 5 references indexed in Scilit:
- Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.Journal of Histochemistry & Cytochemistry, 1981
- Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method.Journal of Histochemistry & Cytochemistry, 1980
- The light microscopic demonstration of hydroperoxidase-positive Phi bodies and rods in leukocytes in acute myeloid leukemiaHistochemistry and Cell Biology, 1978
- Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique.1974