Determination of chloramphenicol residues by reverse phase high performance liquid chromatography infoies gras

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Abstract
A reverse phase liquid Chromatographic (LC) method for the determination of chloramphenicol (CAP) residues in foies gras was tested. The method was based on acetonitrile‐sodium chloride extraction, sample clean‐up with hexane and purification on a Sep‐Pak C18 cartridge. Acceptable Chromatographic separation was obtained for thawed samples with two sample clean‐up steps with hexane, a purification step with acetonitrile + water (87.5 + 12.5) (v/v) before the elution of CAP residues by a mixture of acetonitrile + water (70 + 30) (v/v) with a Chromatographie separation on Spherisorb ODS‐2 (5 μm) column using sodium acetate buffer (pH 4.3) + acetonitrile (78 + 22) (v/v) as mobile phase. The detection limit of the method was 2.5 μg/kg. The mean recovery from spiked samples at the 10 μg/kg level was 55.7 ± 8.7%.