A Study of the Role of Protein Synthesis in the Stimulation of Acid Secretion by Gastrin and a Gastrin-like Pentapeptide

Abstract
Gastric mucosa of the bullfrog was incubated in vitro in the presence of puromycin and actinomycin D. Stimulation of acid secretion by porcine gastrin and a synthetic gastrin-like pentapeptide was not altered by these inhibitors of protein synthesis. Puromycin but not actinomycin D markedly inhibited the incorporation of C14-leucine into mucosal protein. New protein synthesis is not required for the acid stimulating action of gastrin and related peptides on the isolated bullfrog gastric mucosa.