Molecular analysis of DLA‐DRBB1 polymorphism
- 1 November 1996
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 48 (5) , 554-561
- https://doi.org/10.1111/j.1399-0039.1996.tb02669.x
Abstract
The polymorphism of the canine major histocompatibility complex class II DRB gene, DRBB1, was analyzed. Exon 2 that encodes the polymorphic 81 domain was amplified by the polymerase chain reaction (PCR). The PCR product from 250 dogs was cloned and sequenced. Eighteen alleles were identified. Most of the variation in amino acid composition occurred at positions in the peptide binding site. Inheritance of these sequences showed Mendelian segregation with one or two alleles per dog. Cluster analysis of the nucleotide and predicted amino acid sequences subdivided the canine DRBB1 alleles into three major allelic groups. The number of nonsynonymous changes was higher than the number of synonymous changes in the putative antigen recognition sites indicative of positive selection. The data generated can serve as a basis for developing a typing assay for the canine DRBB1 gene.Keywords
This publication has 15 references indexed in Scilit:
- HLA Class II region nucleotide sequences, 1995Tissue Antigens, 1995
- Cloning and sequencing of expressed DRB genes of the red deer (Cervus elaphus) MhcImmunogenetics, 1995
- Class II B Mhc Motifs in an Evolutionary PerspectiveImmunological Reviews, 1995
- ?Both man & bird & beast?: comparative organization of MHC genesImmunogenetics, 1995
- Three-dimensional structure of the human class II histocompatibility antigen HLA-DR1Nature, 1993
- Cloning and sequence analysis of 14 DRB alleles of the bovine major histocompatibility complex by using the polymerase chain reactionAnimal Genetics, 1991
- Allelic variation in theDR subregion of the canine major histocompatibility complexImmunogenetics, 1990
- Nucleotide substitution at major histocompatibility complex class II loci: evidence for overdominant selection.Proceedings of the National Academy of Sciences, 1989