FRACTIONATION OF HIGH MOLECULAR WEIGHT POLYPEPTIDES FROM BEER USING TWO DIMENSIONAL GEL ELECTROPHORESIS

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Abstract
High molecular weight polypeptides from beer were fractionated by a two dimensional gel electrophoretic method comprising isoelectric focusing in the first dimension followed by gradient gel electrophoresis in the second dimension. Silver stained gels revealed a complex pattern of spots which is consistent with individual polypeptide species having both discrete isoelectric points and molecular weights. The pattern of silver stained spots was reproducible both within and between beers and was independent of the sample preparation method or the composition of the sample solubilisation mix. Beers produced from grists containing malt plus 20% wheat flour were shown to contain four polypeptides of approximate molecular weight 30 000, 30 000, 16 000 and 15 000 which were absent from all malt control beers.