Purification and characterization of acidic fibroblast growth factor from bovine brain.
- 1 January 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (2) , 357-361
- https://doi.org/10.1073/pnas.81.2.357
Abstract
Acidic brain fibroblast growth factor was purified a minimum of 35,000-fold to apparent homogeneity by a combination of differential salt precipitation, ion exchange chromatography, gel filtration, isoelectric focusing and hydrophobic chromatography on a C4 reversed-phase HPLC [high performance liquid chromatography] column. Two microheterogeneous forms of the molecule are obtained with apparent MW of 16,600 and 16,800 daltons. The mitogen is highly active with half-maximal stimulation of BALB/c 3T3 fibroblasts at .apprx. 40 pg/ml in an assay using incorporation of [methyl-3H]thymidine into DNA.Keywords
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