The Effect of Chlorine in Water on Enteric Viruses. II. The Effect of Combined Chlorine on Poliomyelitis and Coxsackie Viruses

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Abstract
Polioviruses and Coxsackie virus in water were inactivated by combined residual chlorine, the effective concentration depending upon pH, contact period, and strains of virus. At 25[degree]C and pH 7, at least 9 ppm were necessary for inactivation of poliovirus within 30 minutes; 6 ppm within 1 hour; 0.5 ppm within 7 hours or more. Decreasing pH decreased rate of inactivation. Differences in resistance to chlorine were found among strains of virus. The inactivation rate of a progeny line which survived chlorination was similar to that of the parent line. The dose of combined chlorine at present recommended for the disinfection of sewage did not inactivate the viruses studied.