Effect of ginseng saponins on nuclear ribonucleic acid (RNA) metabolism. II. RNA polymerase activities in rats treated with ginsenoside.
- 1 January 1979
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 27 (9) , 2130-2136
- https://doi.org/10.1248/cpb.27.2130
Abstract
The activities of RNA polymerases I, II and III in a sodium deoxycholate(DOC)-treated enzyme preparation were differentially determined using 3 combinations of assay conditions (metal ion; Mg2+ or Mn2+, (NH4)2SO4 and .alpha.-amanitin). Ginsenoside-Rb1 enhanced, while -Rc repressed, the activities of RNA polymerases I and II; both had no effect on that of RNA polymerase III. Rb1-treated rats showed different profiles of stimulation of RNA polymerases I and II; the maximum increase of RNA polymerase I activity was .apprx. +70% at 2 h, and that of RNA polymerase II activity was +40% at 3 h after the injection of Rb1. Actinomycin D and cycloheximide both blocked the increase in RNA polymerase I activity on treatment with ginsenoside-Rb1. Increased activity of RNA polymerase II was blocked by actinomycin D but not by cycloheximide. Apparently there is transcriptional regulation in the enhancement by ginsenoside-Rb1 of RNA polymerase activities I and II, though the mechanisms may differ in detail.This publication has 9 references indexed in Scilit:
- Phosphorylation of calf thymus RNA polymerase II by nuclear cyclic 3′,5′-AMP-independent protein kinaseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
- Enhancement of formation of the initiation complex by a factor stimulating RNA polymerase II from Ehrlich ascites tumor cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
- Phosphorylative and functional modifications of nucleoplasmic RNA polymerase II by homologous adenosine 3':5'-monophosphate-dependent protein kinase from calf thymus and by heterologous phosphatase.Journal of Biological Chemistry, 1977
- A protein cofactor that stimulates the activity of DNA-dependent RNA polymerase I on double-stranded DNABiochemistry, 1977
- Effects of α-amanitin, cycloheximide, and thioacetamide on low molecular weight nuclear RNABiochemistry, 1976
- Effect of ginseng saponins on nuclear ribonucleic acid (RNA) metabolism. I. RNA synthesis in rats treated with ginsenosides.CHEMICAL & PHARMACEUTICAL BULLETIN, 1976
- Synthesis of Ribonucleic Acid during Early Estrogen ActionJournal of Biological Chemistry, 1968
- Increased template activity of liver chromatin, a result of hydrocortisone administration.Proceedings of the National Academy of Sciences, 1965
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951