Facile Release of NADPH Oxidase from Polymorphonuclear Leukocyte Membrane by Mild Pressure Treatment

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Abstract
NADPH oxidase, a complex enzyme system in the cell membrane responsible for the bactericidal function of polymorphonuclear leukocytes through the production of superoxide anion, was facilely released by mild treatment with a press. At the pressure where almost all NADPH oxidase activity was released, releases of the activities of lactate dehydrogenase, 5′-nucleotidase, lysozyme, and N-acetyl-β- glucosaminidase, and of the amount of total protein were negligible. This method can be useful for the elucidation of NADPH oxidase.