Growth Characteristics of Anaerobically Treated Ehrlich Ascites Tumor Cells after Reaeration as Studied by Combination of Flow Cytometry and Centrifugal Elutriation

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Abstract
Centrifugal elutriation was applied to separate into cell compartments asynchronous [mouse] Ehrlich ascites cells grown under different culture conditions. The cytokinetic properties of the recultivated fractions were studied by flow cytometry. The present experiments prove that G1-cohorts grown 12 h under exclusion of oxygen accumulate in the late G1-compartment. The cytokinetic properties of these cells are not changed. The cytokinetic properties of anaerobically treated S-phase cells are changed; most of S-cells leave the cell cycle after they have attained the DNA content of G2-cells and continue to synthesize DNA without preceding division. Cells with a DNA content up to the 4-fold of normal values are found.