Comparative studies of the immunogenic activity of hepatitis B surface antigen (HBsAg) and hbsag polypeptides

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Abstract
Three hepatitis B surface antigen (HBsAg) preparations were compared: Purified intact 22‐nm HBsAg particles; HBsAg‐derived, sodium dodecyl sulfate (SDS)‐denatured P25 + GP30 polypeptide pool; and nondenatured P25 + GP30 micelles. The micelles had the same polypeptide composition as the P25 + GP30 pool. The immunogenicity in mice of each preparation, administered either in saline suspension or adsorbed to aluminum gel, was compared. The SDS‐denatured polypeptides were less immunogenic than intact HBsAg particles, whereas the micelles were more immunogenic. High anti‐HBs titers were observed in mice immunized with micelle preparations in either saline suspension or adsorbed to aluminum gel for as long as 200 days after a booster inoculation, administered 26 days after the primary dose.