Reconstitution of 5-hydroxytryptamine transport from cholate-disrupted platelet plasma membrane vesicles
- 1 November 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (24) , 5300-5303
- https://doi.org/10.1021/bi00617a033
Abstract
The bile acid cholate disrupted [human] platelet plasma membrane vesicles and inactivated 5-hydroxytryptamine transport activity. The transporter appeared not to be solubilized by this procedure, but remained associated with a high MW aggregate. Merely removing cholate did not restore the vesicular structure or transport activity, but in the presence of added soybean phospholipids, cholate removal generated proteoliposomes which accumulated 5-hydroxytryptamine. This reconstituted transport activity was similar in its ionic requirements and inhibitor sensitivity to transport in native membrane vesicles and intact platelets.This publication has 9 references indexed in Scilit:
- Platelet 5-hydroxytryptamine transport, an electroneutral mechanism coupled to potassiumBiochemistry, 1978
- Studies of the beta-galactoside transporter in inverted membrane vesicles of Escherichia coli. I. Symmetrical facilitated diffusion and proton gradient-coupled transport.Journal of Biological Chemistry, 1977
- Reconstitution and purification of the D-glucose transporter from human erythrocytes.Journal of Biological Chemistry, 1977
- The effects of alterations of transmembrane Na+ and K+ gradients by ionophores (nigericin, monensin) on serotonin transport in human blood plateletsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Active transport of 5-hydroxytryptamine by plasma membrane vesicles isolated from human blood platelets.Journal of Biological Chemistry, 1977
- Single bilayer vesicles prepared without sonication physico-chemical propertiesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Reconstitution of specific Na+-dependent D-glucose transport in liposomes by Triton X-100 extracted proteins from purified brush border membranes of hamster small intestineBiochemical and Biophysical Research Communications, 1976
- CYTOCHEMISTRY AND ELECTRON MICROSCOPYThe Journal of cell biology, 1963
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951