Effect of adenosine 3',5'-monophosphate on growth and several functions of cultured mastocytoma P-815 cells.

Publisher Website
Google Scholar
Abstract
Growth-inhibited mouse mastocytoma P-815 cells at stationary phase contained more histamine, serotonin and c[cyclic]AMP, and higher activities of histidine decarboxylase and adenylate cyclase than the cells during exponential growth. The elevation of endogenous cAMP levels, induced by several growth-inhibiting agents such as N6 O2''-dibutyryl, cAMP (Bt''2cAMP), prostaglandin E1, AMP and 2-chloroadenosine, stimulated several functions characteristic of mastocytoma P-815 cells in culture, elevating the synthesis of histamine and serotonin, the activity of chymotrypsin-like protease, and the incorporation of [35S]sulfate into acidic glycosaminoglycans. 1-Methyl-3-isobutyl-xanthine (MIX), a potent inhibitor of cAMP phosphodiesterase, potentiated the simulatory effect of these agents. cAMP probably regulates the growth and functions of mastocytoma P-815 cells. [35S]-Sulfated acidic glycosaminoglycans synthesized in cells at stationary phase or in cells treated with Bt2cAMP plus MIX mainly localized in the 3000-10,000 .times. g sedimentable fraction of cell homogenates, and had a MW of 200,000 to 400,000 based on gel filtration. This acidic glycosaminoglycan was resistant to chondroitinase ABC and the heparin-degrading enzyme present in the 20,000 .times. g sedimentable fraction of the cells, and was identified as a highly sulfated macromolecular heparin based on behaviors on DEAE-cellulose column and on acidic electrophoresis. Cycloheximide suppressed the stimulatory effect of Bt2cAMP on the synthesis of histamine and [35S]-sulfated acidic glycosaminoglycan.

This publication has 4 references indexed in Scilit: