Nuclear Magnetic Resonance Structure of the Varkud Satellite Ribozyme Stem−Loop V RNA and Magnesium-Ion Binding from Chemical-Shift Mapping,

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Abstract
An important step in the substrate recognition of the Neurospora Varkud Satellite (VS) ribozyme is the formation of a magnesium-dependent loop/loop interaction between the terminal loops of stem−loops I and V. We have studied the structure of stem−loop V by nuclear magnetic resonance spectroscopy and shown that it adopts a U-turn conformation, a common motif found in RNA. Structural comparisons indicate that the U-turn of stem−loop V fulfills some but not all of the structural characteristics found in canonical U-turn structures. This U-turn conformation exposes the Watson−Crick faces of the bases within stem−loop V (G697, A698, and C699) and makes them accessible for interaction with stem−loop I. Using chemical-shift mapping, we show that magnesium ions interact with the loop of the isolated stem−loop V and induce a conformational change that may be important for interaction with stem−loop I. This study expands our understanding of the role of U-turn motifs in RNA structure and function and provides insights into the mechanism of substrate recognition in the VS ribozyme.