Fluorescence Quantum Yields of Tryptophan and Tyrosine
- 1 January 1967
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 1 (1) , 35-42
- https://doi.org/10.1080/00032716708051097
Abstract
Fluorescence quantum yields (Q) for tyrosine, tryptophan, and phenylalanine in water at 23° determined by the comparative method using a quinine standard were found to be 0.14, 0.13, and 0.024, respectively. Similar values were obtained with phenol as the standard. The numbers are much lower than those literature values which have been widely used to calculate protein fluorescence quantum yields. Possible reasons for the discrepancies are discussed. The quantum yields of isomeric tyrosines and fluorophenylalanines are also reported.Keywords
This publication has 16 references indexed in Scilit:
- Practical aspects of the calibration and use of the Aminco-Bowman spectrophotofluorometerAnalytical Biochemistry, 1967
- Some characteristics of the fluorescence of quinineAnalytical Biochemistry, 1967
- Fluorescence Quantum Yield of I-Dimethyl-aminonaphthalene-5-sulphonateNature, 1966
- Fluorescence Quantum Yield Measurements: Vitamin B 6 CompoundsScience, 1965
- Fluorescence assay of high concentrations of DPNH and TPNH in a spectrophotofluorometerAnalytical Biochemistry, 1965
- Correction of Luminescence Spectra and Calculation of Quantum Efficiencies Using Computer Techniques.Analytical Chemistry, 1963
- Correction of fluorescence spectra and measurement of fluorescence quantum efficiencyThe Analyst, 1960
- Ultraviolet fluorescence of the aromatic amino acidsBiochemical Journal, 1957
- Determination of the absolute quantum yield of fluorescent solutionsTransactions of the Faraday Society, 1957
- Fluorescence of some proteins, nucleic acids and related compoundsArchives of Biochemistry and Biophysics, 1956