Genes for the plastid elongation factor Tu and ribosomal protein S7 and six tRNA genes on the 73 kb DNA from Astasia longa that resembles the chloroplast DNA of Euglena
- 1 February 1990
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 220 (3) , 425-432
- https://doi.org/10.1007/bf00391749
Abstract
The nucleotide sequence of a 6156 by segment of the circular 73 kb DNA from Astasia longa resembling the chloroplast DNA of Euglena was determined. The genes for the plastid elongation factor Tu (tufA) and the ribosomal protein S7 (rps7), six tRNA genes (trnQ, trnS, trnG, trnM, trnT, trnR), and three open reading frames were identified. These genes show a high degree of sequence similarity (73%–99%) to the corresponding genes on the Euglena chloroplast genome. The tufA gene contains two small AT-rich introns within its coding region. Northern analysis revealed the in vivo transcription of the tufA gene and of a reading frame of 456 codons into monocistronic mRNAs of 1.3 and 1.4 kb, respectively. The arrangement and organization of the genes on the 73 kb DNA of the colourless heterotrophic flagellate Astasia and the chloroplast DNA of autotrophic Euglena are compared.This publication has 34 references indexed in Scilit:
- The plastid rpoA gene encoding a protein homologous to the bacterial RNA polymerase alpha subunit is expressed in pea chloroplastsMolecular Genetics and Genomics, 1989
- Genes for the ribosomal proteins S12 and S7 and elongation factors EF-G and EF-Tu of the cyanobacterium, Anacystis nidulans: Structural homology between 16S rRNA and S7 mRNAMolecular Genetics and Genomics, 1989
- Occurrence and spacing of ribosome recognition sites in mRNAs of chloroplasts from higher plantsFEBS Letters, 1988
- Control of plastid gene expression: 3′ inverted repeats act as mRNA processing and stabilizing elements, but do not terminate transcriptionCell, 1987
- Organization and expression of the chloroplast genomePlant Science, 1987
- Euglena gracilis chloroplast DNA: analysis of a 1.6 kb intron of the psb C gene containing an open reading frame of 458 codonsCurrent Genetics, 1986
- Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNANature, 1986
- Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNADNA, 1985
- Rapid and Sensitive Protein Similarity SearchesScience, 1985
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977