Selective Extraction of the DM‐20 Brain Proteolipid

1 January 1986

journal article
Published by Wiley in Journal of Neurochemistry

Vol. 46 (1) , 297-299
https://doi.org/10.1111/j.1471-4159.1986.tb12962.x

Abstract

Brain DM‐20 proteolipid was previously shown to be structurally different from the myelin major proteo‐lipid (MMPL). In an attempt to set up a large‐scale purification of DM‐20, we studied extraction of brain proteo‐lipids with mixtures of methylene chloride containing up to 80% methanol. The CH₂Cl₂‐CH₃OH (3:7, vol/vol) mixture is highly selective for the extraction of bovine brain DM‐20 compared to MMPL. Purified DM‐20 was obtained after chromatography of the extract on methylated Sephadex.

Keywords

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