Radiochromium-Labeled Lymphocytes in the Rat.

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Abstract
Summary A technic is described for labeling rat lymphocytes, collected from intestinal lymph, with radiochromium without impairing their motility. After intravenous administration, these labeled cells disappeared rapidly from the circulating blood, reappeared to a limited extent within 6 hours, then continued to disappear at a very slow rate (about 1.1% per hour). When the rat's intestinal lymphatic trunk was cannulated, 4 to 7% of the labeled lymphocytes appeared in the lymph within 4 days, with maximal lymphatic radioactivity occurring between the 24th and 36th hours.
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