Identification of Homologous Ribosomal Proteins in HeLa Ceils and in Tetrahymena pyriformis. A Study of Proteins Binding 5-S RNA and Acidic Proteins Released from 40-S Subunits by EDTA

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Abstract
T. pyriformis 60S ribosomal subunits treated with EDTA release a 7S particle containing 5S RNA and a 36,000-MW protein that is similar to mammalian 5S-RNA-binding protein L5 in MW, in 2-dimensional acrylamide gel mobility and in peptide pattern as generated by a simple, 1-dimensional acrylamide gel technique. Human and T. pyriformis 40S ribosomal subunits, treated with buffers lacking Mg or containing EDTA, release varying amounts of 2 large acidic proteins. These released proteins were identified by 2-dimensional gel electrophoresis.