Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR
Open Access
- 1 March 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (3) , 1155-1156
- https://doi.org/10.1128/jcm.39.3.1155-1156.2001
Abstract
A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/μg of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.Keywords
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