Genetics ofAspergillus flavus: complementation and mapping of aflatoxin mutants

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Abstract
Mutants of Aspergillus flavus impaired in aflatoxin production were induced with N-methyl-N′-nitrosoguanidine and analysed by means of the parasexual cycle. The gene symbol afl was assigned to this type of mutation. Diploid complementation tests revealed that most of 14 afl mutants belonged to different complementation groups. One mutant (afl-1) failed to complement or only partially complemented all other mutants. Haploidization of one diploid revealed the independent segregation of two afl mutants. Heterozygous diploids were synthesized between two afl mutants and tester strains genetically labelled on eight linkage groups. Haploidization of the diploids led to the assignment of two aft mutants to linkage groups. Linkage of afl-4 to w+ and afl-1 to leu on linkage groups II and VII, respectively, was demonstrated.