Unique species of mRNA from adenovirus type 7 early region 1 in cells transformed by adenovirus type 7 DNA fragment

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Abstract
Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I .cntdot. J fragment (the left 7.8% of the viral genome) and in human [oral carcinoma] KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the early stage of productive infection, 2 mRNA (950 and 840 nucleotides long) with the common 5'' and 3'' ends but different internal splicings were mapped from region 1A (map units 1.4-4.3), and 1 mRNA (2310 nucleotides long, with the internal splicing between map units 9.9-10.1) was mapped from region 1B (map units 4.6-11.4). At the late stage, these early spliced mRNA were also found and at least 3 additional Ad7 mRNA were identified: 700 nucleotide-long mRNA in region 1A; and 1100 and 700 nucleotide-long mRNA in region 1B. In transformed rat cell lines, 2 early region 1A mRNA (950 and 840 nucleotides long) were also transcribed. Surprisingly, in addition, several unique Ad7 mRNA, not found in productively infected cells, were identified in all of the transformed cell lines. Their molecular sizes and coding sequences varied in individual cell lines. These mRNA had the 5'' end-proximal portion in region 1B and the 3'' end-proximal portion in region 1A, these portions being transcribed by extending from region 1B to 1A on viral DNA fragments joined in a tandem array in transformed cells.