Isozyme Analysis and Aminopeptidase Activities Within the GenusPeronosclerospora

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Abstract
Electrophoresis and aminopeptidase assays were used to study the taxonomy within the genus Peronosclerospora. The banding patterns of 26 enzymes were determined by horizontal starch gel electrophoresis, and coefficients of similarity were calculated among isolates of P. sorghi, P. sacchari, and P. philippinensis. Isolates of P. sorghi from Thailand were not closely related to any other isolate in this study. Isolates of P. sacchari and P. philippinensis exhibited identical phenotypes for 22 enzymes and probably represent a single species. P. maydis shared phenotypes with P. sacchari and the Thailand isolates but also displayed unique banding patterns in certain enzyme systems. Electrophoresis readily separated isolates of Peronosclerospora from those of Bremia lactucae and Peronospora tabacina. Aminopeptidase assays could not differentiate isolates of P. sorghi, P. sacchari, and P. philippinensis due to large amounts of intraspecific variation.