Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
Top Cited Papers
- 1 December 2009
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 75 (23) , 7537-7541
- https://doi.org/10.1128/aem.01541-09
Abstract
Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.Keywords
This publication has 30 references indexed in Scilit:
- A core gut microbiome in obese and lean twinsNature, 2008
- The Ribosomal Database Project: improved alignments and new tools for rRNA analysisNucleic Acids Research, 2008
- Molecular Characterization of the Diversity and Distribution of a Thermal Spring Microbial Community by Using rRNA and Metabolic GenesApplied and Environmental Microbiology, 2008
- SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARBNucleic Acids Research, 2007
- The Human Microbiome ProjectNature, 2007
- Community Structure Analyses Are More Sensitive to Differences in Soil Bacterial Communities than Anonymous Diversity IndicesApplied and Environmental Microbiology, 2006
- Introducing SONS, a Tool for Operational Taxonomic Unit-Based Comparisons of Microbial Community Memberships and StructuresApplied and Environmental Microbiology, 2006
- Microbial diversity in the deep sea and the underexplored “rare biosphere”Proceedings of the National Academy of Sciences, 2006
- Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARBApplied and Environmental Microbiology, 2006
- NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genesNucleic Acids Research, 2006