A protocol for the routine measurement of lactate and pyruvate in cord blood

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Abstract
Summary. The influence of the site of sampling and the delay in sample collection on cord blood concentrations of lactate and pyruvate were investigated. A delay in sample collection of >1 min after cord clamping could invalidate the results obtained. Samples from umbilical artery had higher lactate values (mean 3·13 mmol/l) than paired vein samples (mean 2·32 mmol/1) but there were no significant differences in lactate values between different sites sampled within the umbilical vein. A calculation was devised to circumvent the need to take cord blood samples immediately after delivery.