Phosphorylation and Activation of p70 s6k by PDK1
- 30 January 1998
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 279 (5351) , 707-710
- https://doi.org/10.1126/science.279.5351.707
Abstract
Activation of the protein p70 s6k by mitogens leads to increased translation of a family of messenger RNAs that encode essential components of the protein synthetic apparatus. Activation of the kinase requires hierarchical phosphorylation at multiple sites, culminating in the phosphorylation of the threonine in position 229 (Thr 229 ), in the catalytic domain. The homologous site in protein kinase B (PKB), Thr 308 , has been shown to be phosphorylated by the phosphoinositide-dependent protein kinase PDK1. A regulatory link between p70 s6k and PKB was demonstrated, as PDK1 was found to selectively phosphorylate p70 s6k at Thr 229 . More importantly, PDK1 activated p70 s6k in vitro and in vivo, whereas the catalytically inactive PDK1 blocked insulin-induced activation of p70 s6k .Keywords
This publication has 14 references indexed in Scilit:
- The modular phosphorylation and activation of p70s6kPublished by Wiley ,1998
- Dual Requirement for a Newly Identified Phosphorylation Site in p70s6kMolecular and Cellular Biology, 1997
- Rapamycin suppresses 5'TOP mRNA translation through inhibition of p70s6kThe EMBO Journal, 1997
- Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase BαCurrent Biology, 1997
- The Principal Rapamycin-Sensitive p70s6k Phosphorylation Sites, T-229 and T-389, Are Differentially Regulated by Rapamycin-Insensitive Kinase KinasesMolecular and Cellular Biology, 1996
- Activation and phosphorylation of a pleckstrin homology domain containing protein kinase (RAC-PK/PKB) promoted by serum and protein phosphatase inhibitors.Proceedings of the National Academy of Sciences, 1996
- Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase BNature, 1995
- Characterization of a Ca2+/Calmodulin-dependent Protein Kinase CascadeJournal of Biological Chemistry, 1995
- Phosphatidylinositol 3-kinase signals activation of p70 S6 kinase in situ through site-specific p70 phosphorylation.Proceedings of the National Academy of Sciences, 1995
- PDGF- and insulin-dependent pp70S6k activation mediated by phosphatidylinositol-3-OH kinaseNature, 1994