Inhibition of repair of premutational lesions in plateau phase Chinese hamster cells exposed to gamma radiation

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Abstract
Induction and repair of forward mutations to 8-azaguanine resistance were studied in gamma irradiated, plateau phase Chinese hamster ovary cells. Mutation induction increased with dose with a relatively low induction for doses below 4 Gy and a steep increase thereafter. A close correlation between the ability of radiation to induce both lethality and mutations in plateau phase cells was evident. Recovery from potentially lethal damage resulted in a significant decrease in mutation frequency suggesting the possible involvement of an error free repair pathway. Mutation response at the end of recovery period was approximately linear with a slope of 2 × 10−5 mutants per viable cells per Gy. This difference as compared to the immediate plating response supports the involvement of two types of damage in the induction of mutations: the nonrepairable, single hit component and a repairable component resulting from the interaction of lesions. Post-irradiation nonlethal hyperthermic treatment (42.5° C; 30 min) sensitized the cells to killing as seen by the thermal enhancement ratio of 1.37. Interaction of hyperthermia, however, did not alter the mutation frequency obtained on immediate plating. Both post-irradiation hyperthermia and incubation at 4° C inhibited most of the recovery from potentially lethal damage and also the repair of premutational lesions. These treatments resulted in a mutation frequency decrease of only 10–15% as compared to 50% seen in cells which actively repaired potentially lethal damage. The temperature dependence for the repair of premutational lesions suggests that the process is mediated by metabolically active steps.

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