ISOLATION IN UNIALGAL CULTURE OF LICHEN GONIDIA BY A SIMPLE PLASMOLYSIS TECHNIQUE
- 1 July 1944
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 19 (3) , 559-561
- https://doi.org/10.1104/pp.19.3.559
Abstract
A method is described whereby gonidial algae may be separated from the lichen thallus and grown on culture media without being contaminated by other algae often casually present on the lichen. Through this technique the gonidial algae from Ramalina complanata and Parmelia perforata were isolated in unialgal culture and grown in soil extract media and in 0.05% Knop''s soln. The method consists of plasmolyzing all surface organisms by immersing the thallus in a 2 M KC1 or MgSO4 soln. for 30 sec., rinsing 5-6 times with sterile dist. water, and lightly grinding the lichen by means of coarsely ground glass in a mortar before inoculation. Liquid culture media gave the most rapid growth.This publication has 1 reference indexed in Scilit:
- ISOLATION IN PURE CULTURE OF GREEN ALGAE FROM SOIL BY A SIMPLE TECHNIQUEPlant Physiology, 1932