Improvement of the Electro-transformation Efficiency of Facultatively Alkaliphilic Bacillus pseudofirmus OF4 by High Osmolarity and Glycine Treatment
- 1 January 2001
- journal article
- Published by Oxford University Press (OUP) in Bioscience, Biotechnology, and Biochemistry
- Vol. 65 (12) , 2773-2775
- https://doi.org/10.1271/bbb.65.2773
Abstract
An electro-transformation method was established for facultatively alkaliphilic Bacillus pseudofirmus OF4. A high osmolarity electroporation medium with a high electric field strength was effective. Transformation efficiency improved 110-fold in glycine-treated cells compared to non-glycine-treated cells. Under optimum conditions, the transformation efficiency was 1.69 x 10(6) transformants per microg of pUB110.Keywords
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