Toxin Production and Proteolytic Activity of Clostridium Botulinum in Peptone and Peptone Egg White Medium

Abstract
With Cl. botulinum, type A, grown in peptone broth and coagulated egg-white peptone medium, the growth curve and toxin titer curve reached a maximum in 48 hrs. When coagulated egg-white peptone medium was used, the formol titration showed greatest proteolysis up to the 10th day, the increase being most rapid during the first 48 hrs.; toxin titer remained constant for 20 days. In peptone broth, toxin titer decreased after 48 hrs. and formol titration decreased after 10 days.

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