EFFECTS OF 5-BROMODEOXYURIDINE IN AUTOTETRAPLOID BARLEY

Abstract
Two F1 hybrid populations of barley (Hordeum vulgare L. emend Lam.) were treated with 5-Bromodeoxyuridine (BUdR), and subsequently the chromosome numbers were doubled with colchicine. In relation to the control plants, the BUdR-treated plants had higher fertility (seed set), a greater percentage of pollen mother cells (PMC) with 14-14 chromosome distribution at anaphase-1, and a greater degree of preferential pairing as measured by the percentage of recessives in the F2 generation. It is proposed, since BUdR causes single base pair changes, that the unit of a chromosome associated with preferential pairing is at least as small as a single base pair.

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