In vitro transcription of viroid RNA into full-length copies by RNA-dependent RNA polymerase from healthy tomato leaf tissue
- 1 March 1982
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 2 (3) , 185-194
- https://doi.org/10.1007/bf01116382
Abstract
RNA-dependent RNA polymerase from healthy tomato plant tissue accepts potato spindle tuber viroid (PSTV) RNA as a template for the in vitro synthesis of full-length RNA copies of the PSTV genome. Viroid transcription requires the presence of Mn2+ and/or Mg2+ ions and is not inhibited by concentrations of 10−5 M α-amanitin. This is the first report of a well-defined product synthesized in vitro by an RNA-dependent RNA polymerase from healthy plants.Keywords
This publication has 25 references indexed in Scilit:
- DNA-dependent RNA polymerase II of plant origin transcribes viroid RNA into full-length copiesNature, 1981
- Detection of complementary RNA intermediates of viroid replication by Northern blot hybridizationBioscience Reports, 1981
- Properties of the complementary RNA sequences associated with infection by the citrus exocortis viroidVirology, 1980
- STRUCTURE AND POSSIBLE FUNCTIONS OF VIROIDSAnnals of the New York Academy of Sciences, 1980
- Tomato DNA contains no detectable regions complementary to potato spindle tuber viroid as assayed by Southern hybridizationVirology, 1980
- Tomato DNA contains no detectable regions complementary to potato spindle tuber viroid as assayed by solution and filter hybridizationVirology, 1980
- Viroid replication is inhibited by α-amanitinNature, 1979
- RNA sequences complementary to citrus exocortis viroid in nucleic acid preparations from infected Gynura aurantiacaProceedings of the National Academy of Sciences, 1978
- Characterization of the Subunits of Qβ ReplicaseNature, 1970
- Subunit Structure of Qβ ReplicaseNature, 1970