Involvement of Azopirillum brasilense plasmid DNA in the productio of indole acetic acid

Abstract
Indole acetic acid (IAA) production in Azospirillum brasilense strain Sp245 is controlled by a 85 MDa plasmid naturally in this bacterium. In the presence of l-tryptophan, anthranilic acid production and almost no IAA production occurs in a derivative strain harbouring a Tn5-Mob insertion in the 85 MDa plasmid. Agrobacterium tumefaciens strain GM19023, upon transfer of Tn5-Mob labelled 85 MDa plamid of A. brasilense Sp245, gains the ability to produce anthranilic acid.

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