cDNA macroarray analysis of gene expression in synoviocytes stimulated with TNFα
- 22 March 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 517 (1-3) , 121-128
- https://doi.org/10.1016/s0014-5793(02)02588-7
Abstract
Gene expression of synoviocytes stimulated with tumor necrosis factor-α (TNFα) was studied by macroarray analysis to elucidate the cellular response and identify new biological functions of known and unknown genes. 10 035 cDNA clones were used to make cDNA macroarrays of representative genes. Synoviocytes expressed large amounts of fibronectin and collagen mRNA. Statistical analysis of the macroarray data revealed 26 genes, including six new genes, which underwent significant alteration of gene expression in response to TNFα stimulation. These findings suggest that the synoviocyte response to TNFα stimulation forms the basis of development of various aspects of the pathophysiology of rheumatoid arthritis.Keywords
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