Preparation of Specific Antisera to Honeybee Viruses by Immunization with Agar Gel Precipitates

Abstract
A technique for the production of specific antisera to sac brood virus (SBV), chronic bee paralysis virus(CBPV) and Kashmir bee virus (KBV) is described. Antisera suitable for gel-diffusion precipitin tests were prepared against SBV, CBPV and KBV by immunizing rabbits with specific agar gel immune precipitates. These precipitates were produced in gel-diffusion precipitin tests using partially purified viral extracts and reference antisera.